Continuing along our previous work to characterize certain cationized glycans with a variety of metals and with cobalt to enhance UV photon absorption, we are pleased to show our effort to demonstrate the formation of heterodimeric cobalt-glycan adducts in published form. Now appearing as an online-first article in Journal of the American Society for Mass Spectrometry, our paper “Assessment of Dimeric Metal-Glycan Adducts via Isotopic Labeling and Ion Mobility-Mass Spectrometry” presents how important ion mobility separations are for tandem MS experiments performed on divalent metal-glycan adducts. Without the ability to use drift times to distinguish which precursor produced which fragment ions, component fragments from a heterodimeric adduct of two isomers cannot be deconvoluted. Specifically, the potential for isomers to form doubly-charged heterodimers–that are then dissociated into component fragments–will essentially create a scenario where chimeric fragmentation spectra are produced.
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