Instrumentation

Group Designed/Built Instrumentation

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Low pressure, variable temperature, ion mobility drift tube design equipped with an ion funnel trap and rear ion funnel for efficient ion transport. For more information on this design see the following manuscript

The core instruments designed and constructed in the Clowers Research Group are focused almost exclusively on the accurate measurement of gas-phase ion properties.  While stand alone ion mobility instruments are extremely useful in a field-based setting the combination of this technique with mass spectrometry provides a second dimension of analysis that is extremely powerful.  The research ion mobility instruments being constructed in our laboratory include both high (760 Torr) and low pressure (~4 Torr) instruments both capable of operating at a range of temperatures.  Below is a rough schematic of our latest stacked-ring IMS system which includes a variable pressure housing to enable a wider range of E/N values to be explored.

APIMS_Assembly

Available ion mobility instruments also include a high pressure ion mobility time of flight instrument capable of performing Hadamard-based multiplexing experiments for high throughput analysis.

AP_IMS_TOF

Commercial Instrumentation

Additionally, the Clowers Research group has a variety of commercial ion mobility spectrometers and mass spectrometers available for a variety of applications. Some of the most utilized instruments in the lab include:

 

 

Three LTQ ion traps, which serve as an analytical workhorse in the laboratory.  It finds uses ranging from standard proteomic experiments and method development. In addition to standard LC-MS experiments, this has now been modified to include a rear optical port compatible with UV photodissociation experiments.  This instrument, which still retains its original functionality, now includes a removable ion mobility drift tube with enables a range of hybrid IMS-MSn experiments combined with photofragmentation.

 

An Agilent 6410 triple-quad mass sepctrometer with an atmospheric flow tube attached to the inlet. This instrument is primarily utilized for studying ion kinetics for the behavioral study of chemical warfare agent simulants.

A Waters Aquity- UPLC-MS which has been used not only to characterize metabolites of N. fowleri in water samples, but also used to analyze samples from inter-departmental collaborations, such as analyzing metabolites in dog plasma and rat brains.

A Bruker prototype TIMS attached to a microTOF III, which is primarily used to bridge the gap between mobility fundamentals of drift tube IMS and trapped ion mobility.

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