Group Designed/Built Instrumentation
The core instruments designed and constructed in the Clowers Research Group are focused almost exclusively on the accurate measurement of gas-phase ion properties. While stand alone ion mobility instruments are extremely useful in a field-based setting the combination of this technique with mass spectrometry provides a second dimension of analysis that is extremely powerful. The research ion mobility instruments being constructed in our laboratory include both high (760 Torr) and low pressure (~4 Torr) instruments both capable of operating at a range of temperatures. Below is a rough schematic of our latest stacked-ring IMS system which includes a variable pressure housing to enable a wider range of E/N values to be explored.
Available ion mobility instruments also include a high pressure ion mobility time of flight instrument capable of performing Hadamard-based multiplexing experiments for high throughput analysis.
Our group has had a long-standing interest in structures for lossless ion manipulations (SLIM) and is happy to report that we are actively engaging in research concerning traveling wave structures for lossless ion manipulations (TW-SLIM). We’ve enjoyed the opportunity to present our TW-SLIM research at numerous conferences and will be publishing a number of articles in the near future.
Additionally, the Clowers Research group has a variety of commercial ion mobility spectrometers and mass spectrometers available for a variety of applications. Some of the most utilized instruments in the lab include:
Three LTQ ion traps, which serve as an analytical workhorse in the laboratory. It finds uses ranging from standard proteomic experiments and method development. In addition to standard LC-MS experiments, this has now been modified to include a rear optical port compatible with UV photodissociation experiments. This instrument, which still retains its original functionality, now includes a removable ion mobility drift tube with enables a range of hybrid IMS-MSn experiments combined with photofragmentation.
An Agilent 6410 triple-quad mass sepctrometer with an atmospheric flow tube attached to the inlet. This instrument is primarily utilized for studying ion kinetics for the behavioral study of chemical warfare agent simulants.
A Waters Aquity- UPLC-MS which has been used not only to characterize metabolites of N. fowleri in water samples, but also used to analyze samples from inter-departmental collaborations, such as analyzing metabolites in dog plasma and rat brains.
A Bruker prototype TIMS attached to a microTOF III, which is primarily used to bridge the gap between mobility fundamentals of drift tube IMS and trapped ion mobility.