{"id":15,"date":"2013-08-13T04:04:33","date_gmt":"2013-08-13T04:04:33","guid":{"rendered":"http:\/\/www.clowersresearch.com\/main\/?page_id=15"},"modified":"2023-03-02T23:44:27","modified_gmt":"2023-03-02T23:44:27","slug":"instrumentation","status":"publish","type":"page","link":"http:\/\/www.clowersresearch.com\/main\/research\/instrumentation\/","title":{"rendered":"Instrumentation"},"content":{"rendered":"\n<h4>Group Designed\/Built Instrumentation<\/h4>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><a href=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2013\/11\/skitch.png\"><img loading=\"lazy\" width=\"1024\" height=\"232\" src=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2013\/11\/skitch-1024x232.png\" alt=\"skitch\" class=\"wp-image-108\" srcset=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2013\/11\/skitch-1024x232.png 1024w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2013\/11\/skitch-300x68.png 300w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2013\/11\/skitch-250x56.png 250w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2013\/11\/skitch-150x34.png 150w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption>Low pressure, variable temperature, ion mobility drift tube design equipped with an ion funnel trap and rear ion funnel for efficient ion transport. For more information on this design see the following <a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC2516354\/\">manuscript<\/a><\/figcaption><\/figure><\/div>\n\n\n\n<p>The core instruments designed and constructed in the Clowers Research Group are focus heavily on the accurate measurement of gas-phase ion properties. &nbsp;While stand alone ion mobility instruments are extremely useful in a field-based setting, the combination of this technique with mass spectrometry provides a second dimension of analysis which is extremely powerful. &nbsp;The research ion mobility instruments being constructed in our laboratory include both high (760 Torr) and <a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC2516354\/\">low pressure<\/a> (~4 Torr) instruments both capable of operating at a range of temperatures. &nbsp;Below is a rough schematic of our latest stacked-ring IMS system which includes a variable pressure housing to enable a wider range of E\/N values to be explored.<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><a href=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2013\/08\/APIMS_Assembly.gif\"><img loading=\"lazy\" width=\"360\" height=\"240\" src=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2013\/08\/APIMS_Assembly.gif\" alt=\"APIMS_Assembly\" class=\"wp-image-196\"\/><\/a><\/figure><\/div>\n\n\n\n<h4>Structures For Lossless Ion Manipulations (SLIM)<\/h4>\n\n\n\n<div class=\"wp-block-jetpack-slideshow aligncenter\" data-effect=\"slide\"><div class=\"wp-block-jetpack-slideshow_container swiper-container\"><ul class=\"wp-block-jetpack-slideshow_swiper-wrapper swiper-wrapper\"><li class=\"wp-block-jetpack-slideshow_slide swiper-slide\"><figure><img loading=\"lazy\" width=\"803\" height=\"1024\" alt=\"\" class=\"wp-block-jetpack-slideshow_image wp-image-1641\" data-id=\"1641\" src=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/Big_board-1-803x1024.jpg\" srcset=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/Big_board-1-803x1024.jpg 803w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/Big_board-1-235x300.jpg 235w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/Big_board-1-768x980.jpg 768w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/Big_board-1-1204x1536.jpg 1204w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/Big_board-1-1606x2048.jpg 1606w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/Big_board-1-91x116.jpg 91w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/Big_board-1-scaled.jpg 2007w\" sizes=\"(max-width: 803px) 100vw, 803px\" \/><\/figure><\/li><li class=\"wp-block-jetpack-slideshow_slide swiper-slide\"><figure><img loading=\"lazy\" width=\"768\" height=\"1024\" alt=\"\" class=\"wp-block-jetpack-slideshow_image wp-image-1642\" data-id=\"1642\" src=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/SLIM_ToF-1-768x1024.jpg\" srcset=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/SLIM_ToF-1-768x1024.jpg 768w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/SLIM_ToF-1-225x300.jpg 225w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/SLIM_ToF-1-1152x1536.jpg 1152w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/SLIM_ToF-1-1536x2048.jpg 1536w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/SLIM_ToF-1-87x116.jpg 87w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/SLIM_ToF-1-scaled.jpg 1920w\" sizes=\"(max-width: 768px) 100vw, 768px\" \/><\/figure><\/li><li class=\"wp-block-jetpack-slideshow_slide swiper-slide\"><figure><img loading=\"lazy\" width=\"1024\" height=\"582\" alt=\"\" class=\"wp-block-jetpack-slideshow_image wp-image-1643\" data-id=\"1643\" src=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/sample_SLIM_boards-1-1024x582.jpg\" srcset=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/sample_SLIM_boards-1-1024x582.jpg 1024w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/sample_SLIM_boards-1-300x170.jpg 300w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/sample_SLIM_boards-1-768x436.jpg 768w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/sample_SLIM_boards-1-1536x873.jpg 1536w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/sample_SLIM_boards-1-2048x1164.jpg 2048w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/sample_SLIM_boards-1-204x116.jpg 204w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure><\/li><\/ul><a class=\"wp-block-jetpack-slideshow_button-prev swiper-button-prev swiper-button-white\" role=\"button\"><\/a><a class=\"wp-block-jetpack-slideshow_button-next swiper-button-next swiper-button-white\" role=\"button\"><\/a><a aria-label=\"Pause Slideshow\" class=\"wp-block-jetpack-slideshow_button-pause\" role=\"button\"><\/a><div class=\"wp-block-jetpack-slideshow_pagination swiper-pagination swiper-pagination-white\"><\/div><\/div><\/div>\n\n\n\n<p>Our group has had a long-standing interest in structures for lossless ion manipulations (SLIM) and are actively engaging in research concerning traveling wave SLIM (TW-SLIM) platforms. Our TW-SLIM research is performed using SLIM boards of varying size and complexity, each of which was designed by members of the Clowers research group. More recently, we have paired one of our larger SLIM boards with Ionicon\u2019s ioniTOF4000 time-of-flight mass spectrometer.<\/p>\n\n\n\n<p><strong>Variable Temperature Drift Tube IMS<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/IMG_8145.jpg\"><img loading=\"lazy\" width=\"961\" height=\"536\" src=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/IMG_8145.jpg\" alt=\"\" class=\"wp-image-1647\" srcset=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/IMG_8145.jpg 961w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/IMG_8145-300x167.jpg 300w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/IMG_8145-768x428.jpg 768w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2023\/03\/IMG_8145-208x116.jpg 208w\" sizes=\"(max-width: 961px) 100vw, 961px\" \/><\/a><figcaption>Our heated FlexTube IMS mounted onto one of our LTQ mass spectrometers.<\/figcaption><\/figure>\n\n\n\n<p>Our group is interested in looking at gas-phase ion chemistry experiments such as ion-neutral clustering and hydrogen\/deuterium exchange (HDX). We developed this heated FlexTube system to suit the applications benefitting from variable temperature experiments. Not pictured, we have machined a case that can be lined with heaters. By combining site-specific ion-neutral clustering and a simultaneous HDX, we obtain data to make inferences on the three dimensional structure of ions as they move through the drift tube.<\/p>\n\n\n\n<h4>Commercial Instrumentation<\/h4>\n\n\n\n<p>Additionally, the Clowers Research group has a variety of commercial ion mobility spectrometers and mass spectrometers available for a variety of applications. Some of the most utilized instruments in the lab include:<\/p>\n\n\n\n<div class=\"mceTemp\">&nbsp;<\/div>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><a href=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2016\/06\/IMG_2414.jpg\"><img loading=\"lazy\" width=\"300\" height=\"225\" src=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2016\/06\/IMG_2414-300x225.jpg\" alt=\"\" class=\"wp-image-417\" srcset=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2016\/06\/IMG_2414-300x225.jpg 300w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2016\/06\/IMG_2414-768x576.jpg 768w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2016\/06\/IMG_2414-1024x768.jpg 1024w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2016\/06\/IMG_2414-200x150.jpg 200w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2016\/06\/IMG_2414-150x112.jpg 150w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/figure><\/div>\n\n\n\n<p>Three LTQ ion traps, which serve as an analytical workhorse in the laboratory. &nbsp;It finds uses ranging from standard proteomic experiments and method development. In addition to standard LC-MS experiments, this has now been modified to include a rear optical port compatible with UV photodissociation experiments. &nbsp;This <a title=\"IMS \u2013 Ion Trap Equipped with UV Photofragmentation\" href=\"http:\/\/www.clowersresearch.com\/main\/ims-ion-trap-equipped-with-uv-photofragmentation\/\">instrument<\/a>, which still retains its original functionality, now includes a removable ion mobility drift tube with enables a range of hybrid IMS-MSn experiments combined with photofragmentation.<\/p>\n\n\n\n<div class=\"mceTemp\">&nbsp;<\/div>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><a href=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2019\/11\/20191104_124643.jpg\"><img loading=\"lazy\" width=\"300\" height=\"225\" src=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2019\/11\/20191104_124643-300x225.jpg\" alt=\"\" class=\"wp-image-927\" srcset=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2019\/11\/20191104_124643-300x225.jpg 300w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2019\/11\/20191104_124643-768x576.jpg 768w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2019\/11\/20191104_124643-1024x768.jpg 1024w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2019\/11\/20191104_124643-200x150.jpg 200w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2019\/11\/20191104_124643-150x113.jpg 150w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/figure><\/div>\n\n\n\n<p>An Agilent 6410 triple-quad mass sepctrometer with an atmospheric flow tube attached to the inlet. This instrument is primarily utilized for studying ion kinetics for the behavioral study of chemical warfare agent simulants.<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><a href=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2016\/12\/IMG_4654-1.jpg\"><img loading=\"lazy\" width=\"300\" height=\"225\" src=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2016\/12\/IMG_4654-1-300x225.jpg\" alt=\"\" class=\"wp-image-440\" srcset=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2016\/12\/IMG_4654-1-300x225.jpg 300w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2016\/12\/IMG_4654-1-768x576.jpg 768w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2016\/12\/IMG_4654-1-1024x768.jpg 1024w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2016\/12\/IMG_4654-1-200x150.jpg 200w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2016\/12\/IMG_4654-1-150x113.jpg 150w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/figure><\/div>\n\n\n\n<p>A Waters Aquity- UPLC-MS which has been used not only to characterize metabolites of <em>N. fowleri<\/em> in water samples, but also used to analyze samples from inter-departmental collaborations, such as analyzing metabolites in dog plasma and rat brains.<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><a href=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2018\/03\/20180130_104735-1-e1521263865666.jpg\"><img loading=\"lazy\" width=\"225\" height=\"300\" src=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2018\/03\/20180130_104735-1-e1521263865666-225x300.jpg\" alt=\"\" class=\"wp-image-556\" srcset=\"http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2018\/03\/20180130_104735-1-e1521263865666-225x300.jpg 225w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2018\/03\/20180130_104735-1-e1521263865666-768x1024.jpg 768w, http:\/\/www.clowersresearch.com\/main\/wp-content\/uploads\/2018\/03\/20180130_104735-1-e1521263865666-113x150.jpg 113w\" sizes=\"(max-width: 225px) 100vw, 225px\" \/><\/a><\/figure><\/div>\n\n\n\n<p>A Bruker prototype TIMS attached to a microTOF III, which is primarily used to bridge the gap between mobility fundamentals of drift tube IMS and trapped ion mobility.<\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Group Designed\/Built Instrumentation The core instruments designed and constructed in the Clowers Research Group are focus heavily on the accurate measurement of gas-phase ion properties. &nbsp;While stand alone ion mobility instruments are extremely useful in a field-based setting, the combination of this technique with mass spectrometry provides a second dimension of analysis which is extremely [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":108,"parent":6,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"http:\/\/www.clowersresearch.com\/main\/wp-json\/wp\/v2\/pages\/15"}],"collection":[{"href":"http:\/\/www.clowersresearch.com\/main\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/www.clowersresearch.com\/main\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/www.clowersresearch.com\/main\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.clowersresearch.com\/main\/wp-json\/wp\/v2\/comments?post=15"}],"version-history":[{"count":9,"href":"http:\/\/www.clowersresearch.com\/main\/wp-json\/wp\/v2\/pages\/15\/revisions"}],"predecessor-version":[{"id":1648,"href":"http:\/\/www.clowersresearch.com\/main\/wp-json\/wp\/v2\/pages\/15\/revisions\/1648"}],"up":[{"embeddable":true,"href":"http:\/\/www.clowersresearch.com\/main\/wp-json\/wp\/v2\/pages\/6"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/www.clowersresearch.com\/main\/wp-json\/wp\/v2\/media\/108"}],"wp:attachment":[{"href":"http:\/\/www.clowersresearch.com\/main\/wp-json\/wp\/v2\/media?parent=15"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}